To measure the entrapment efficiency we use UV not HPLC, is it enough to measure absorbance at any wavelength selected by me? one with max. absorbance for the extract, but not related to a certain compound coz we are not using a standard.
Dear Samar,
thank you for posting this interesting technical question. A formula for calculating the encapsulation efficiency (after UV measurements) can be found in the following potentially useful article:
Development and Characterization of Nanoparticles for the Delivery of Gemcitabine Hydrochloride
This paper has been published Open Access so that it is freely available on the internet (please see attached pdf file).
In this context please also have a look at the answer given to the following closely related questions which have been posted earlier on RG:
How do I calculate encapsulation efficiency?
https://www.researchgate.net/post/How_do_I_calculate_encapsulation_efficiency
(35 answers)
and
What is the best way to determine the entrapment efficiency of siRNA loaded nanoparticles?
https://www.researchgate.net/post/What_is_the_best_way_to_determine_the_entrapment_efficiency_of_siRNA_loaded_nanoparticles
(15 answers)
I hope this information is useful for you. Good luck with your work!
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