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Hi, I have snap-frozen (isopentan-bath) mouse kidneys stored at -80°C and embedded in OCT. How can I de-embedd them to use for a Nephrin ELISA? Thanks.
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Hello there, I want to snap-freeze mouse kidneys for immunofluorescence using isopentane surrounded by liquid nitrogen (1/4 of kidney for 1 min and then rapidly in -80°C freezer). Is there...
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