Konstantin Gradel

3 Questions 1 Answers 0 Followers

Questions related from Konstantin Gradel

De-embedding of OCT kidneys?

Hi, I have snap-frozen (isopentan-bath) mouse kidneys stored at -80°C and embedded in OCT. How can I de-embedd them to use for a Nephrin ELISA? Thanks.

08 August 2019 5,328 1 View

Snap-freezing in isopentane bath with(out) OCT?

Hello there, I want to snap-freeze mouse kidneys for immunofluorescence using isopentane surrounded by liquid nitrogen (1/4 of kidney for 1 min and then rapidly in -80°C freezer). Is there...

27 July 2019 5,029 2 View

Snap-freezing in isopentane bath with(out) OCT?

Hello there, I want to snap-freeze mouse kidneys for immunofluorescence using isopentane surrounded by liquid nitrogen (1/4 of kidney for 1 min and then rapidly in -80°C freezer). Is there...

07 July 2019 9,790 2 View