Data analysis software for high throughput screening of hydrolytic enzymes in metagenomic DNA library?

More Farah Fadwa Ben belgacem's questions See All

Has anyone faced background fluorescence problem of enzymes fluorogenic substrates?

While using 3 fluorogenic substrates derived from Coumarins in cellulose- and xylan-degrading enzymes screening, one of them (MUGlc) shows blue background fluorescence during enzyme assay. is...

31 December 2018 421 3 View

How to interpret such results of FPLC using HIS-tag column?

For my recombinant enzymes, I am not able to see any peak in the elution side of the FPLC results using his-tag column, while a peak is present before elution. is anyone able to interpret what...

07 August 2018 9,224 2 View

Need for Q1 Journal suggestion?

I am about to finish a manuscript about High-throughput screening of biocatalysts from metagenomic DNA library, I am in search of a Q1 journal with no limited words, pages or figures where I can...

03 April 2018 1,870 0 View

How do you define Novel Enzyme?

What are parameters that decide the novelty of an enzyme before characterize it? means If we have a protein sequence which shows certain similarity to a protein in the database, what percentage...

01 February 2018 5,719 11 View

Colony PCR and normal PCR?

I tried to do multiplex colony PCR, I pooled many clones into one tube and I run colony PCR and I did not get bands. To make sure that the problem is not my primers' design, I have extracted the...

31 December 2017 9,074 18 View

Solvent Accessible Surface Area SASA results interpretation?

How can we interpret SASA results when compare between Enzyme and Enzyme-ligand complex?

31 December 2017 2,544 0 View

How to decide the type of a protein from different families given in Blastx?

I have faced this with more than one gene. in the step of gene annotation of my NGS data I found in many case my enzyme of interest but I found that this enzyme is found in multispecies, it means...

11 December 2017 9,593 1 View

Would you please propose a software to compare NGS data to CDD?

After proceeding with Prodigal for Genes prediction we would able to blast it and find known genes in our data. Is it possible to find novel genes by comparing it to conserved domain database CDD?...

11 December 2017 4,004 7 View

Is there any substitute of FosmidMax DNA isolation Kit ?

Is there any manual way to isolate recombinant fosmid DNA from E.coli cells in the absence of isolation Kit?

03 April 2017 3,765 4 View

What is the best choice of Polymerase for large DNA PCR?

I am planning to amplify a large DNA sequence of 40kb with PCR. Can I use a normal Taq Polymerase? Is there any specific one for large sequences amplification?

11 December 2016 8,893 30 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Why 3 replicates for most biological assays? Is it enough to examine the data fits normal distribution?

Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...

31 July 2024 8,141 13 View

Where to find a gene list for CRISPRa/i library screening of regulatory factors that affect pathogenic Th17 differentiation in PBMC?

I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...

31 July 2024 2,150 0 View

Dr Faizul Nisha

Dear Farah,

Kindly go through these links"

1.Statistical Methods for Analysis of High-Throughput RNA Interference Screens.http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2789971/

2.Statistical practice in high-throughput screening data analysis.http://www.nature.com/nbt/journal/v24/n2/full/nbt1186.html

3.Data Analysis Approaches in High Throughput Screening.http://cdn.intechopen.com/pdfs-wm/41155.pdf

Farah Fadwa Ben belgacem

Thank you very much Faizul.

Shuichi Shinmura

I am not sure if my Matroska feature selection method for microarray data will help your research. Check my papers on RG.

Thank you very much prof Shuichi Shinmura