Has anyone faced background fluorescence problem of enzymes fluorogenic substrates?

While using 3 fluorogenic substrates derived from Coumarins in cellulose- and xylan-degrading enzymes screening, one of them (MUGlc) shows blue background fluorescence during enzyme assay. is...

31 December 2018 293 3 View

How to interpret such results of FPLC using HIS-tag column?

For my recombinant enzymes, I am not able to see any peak in the elution side of the FPLC results using his-tag column, while a peak is present before elution. is anyone able to interpret what...

07 August 2018 9,062 2 View

Need for Q1 Journal suggestion?

I am about to finish a manuscript about High-throughput screening of biocatalysts from metagenomic DNA library, I am in search of a Q1 journal with no limited words, pages or figures where I can...

03 April 2018 1,728 0 View

How do you define Novel Enzyme?

What are parameters that decide the novelty of an enzyme before characterize it? means If we have a protein sequence which shows certain similarity to a protein in the database, what percentage...

01 February 2018 5,553 11 View

Colony PCR and normal PCR?

I tried to do multiplex colony PCR, I pooled many clones into one tube and I run colony PCR and I did not get bands. To make sure that the problem is not my primers' design, I have extracted the...

31 December 2017 8,914 18 View

Solvent Accessible Surface Area SASA results interpretation?

How can we interpret SASA results when compare between Enzyme and Enzyme-ligand complex?

31 December 2017 2,416 0 View

How to decide the type of a protein from different families given in Blastx?

I have faced this with more than one gene. in the step of gene annotation of my NGS data I found in many case my enzyme of interest but I found that this enzyme is found in multispecies, it means...

11 December 2017 9,448 1 View

Would you please propose a software to compare NGS data to CDD?

After proceeding with Prodigal for Genes prediction we would able to blast it and find known genes in our data. Is it possible to find novel genes by comparing it to conserved domain database CDD?...

11 December 2017 3,882 7 View

Is there any substitute of FosmidMax DNA isolation Kit ?

Is there any manual way to isolate recombinant fosmid DNA from E.coli cells in the absence of isolation Kit?

03 April 2017 3,621 4 View

What is the maximum duration of storing bacterial library in 20% glycerol stock?

For how many time can we store bacteria in glycerol stock in -80 degree? Do we have to transfer the bacteria to fresh media with glycerol after an approximate duration? In addition to Freeze-Thaw...

11 December 2016 7,297 22 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View