Hi,

I got this result from a LightCycler 96 using TaqMan Master mix and a FAM labelled probe. I highlighted one of the curves (well C6), I hope it's visible. It moves up from the baseline very early, so falsely the software gives it a Cq value of 14.78! Moving the treshold doesn't help in this case, I think the curve should be moved downwards to the others to get a realistic Cq. Is there a way to do this in the software? The RotorGene Q software does this nicely and so much more.

How can I analyze my data properly?

What could be the cause of the higher signal in C6?

Thanks for your help!