Dark object subtraction in google earth engine?

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Content Analysis tool for risk disclosure in annual reports?

hi, can someone help with possible ways of doing content analysis from annual reports of Indian companies. I am looking at risk disclosure practices of companies and how can i create a risk...

13 July 2023 1,075 3 View

CRISPR Cas9 based single nucleotide change in rice?

Can anyone tell me is there any way by which a single base can be changed in rice by using CRISPR Cas9

01 April 2023 5,222 0 View

During conventional stickey end cloning, dies the ratio of vector to insert depends on the size of the vector and insert?

I am trying to clone h 533 bp fragment in a 21000bp vector. What should be appropriate ratio of vector to insert to use for successful cloning.

31 December 2022 6,736 3 View

Which polymerase is good for amplifying 4.5kb fragment from a plasmid?

28 December 2022 9,065 5 View

What is the maximum size of amplicon amplified by pfu polymerase?

I want to amplify a 4.5kb fragment from a plasmid. Can pfu polymerase from genebio is suitable for the job?

28 December 2022 1,412 3 View

How to lyophilize isolated DNA sample in a simple way?

I have DNA isolated from Leishmania parasites using a Qiagen kit. Please guide me how to lyophilize this DNA sample.

08 November 2022 292 2 View

Is it necessary to use T4 polynucleotide kinase during annealing oligos for guide RNA cloning?

I am having difficulties in ligating guide RNA in a vector, digestion happens but ligation doesn't. I am not using T4 polynucleotide kinase during oligo annealing ( Zhang's lab protocol). So, I...

24 September 2022 7,248 0 View

Does anyone know GFP fusion with gene of interest vector for wheat?

I am looking for a vector which allows translational fusion of gene of interest with GFP for wheat. Does anyone know about any such vector.

13 September 2022 6,204 0 View

Golden Gate cloning using Bbs1 not working?

I am trying to assemble 5 different construct in a single destination vector but my digestion ligation reaction is not working. I am getting all blue colonies except one or two white colonies. By...

10 July 2022 359 0 View

What is the formula to calculate Canopy cover % ? We have diameter at breast height, crown area of individual trees, total number of trees, and area ?

I am looking for a good formula to compute % canopy cover for the trees of a plot. I have the dbh for all the trees, calcualted crown projection area (using the crown radius of the species),...

10 May 2022 1,939 0 View

Do you know best conditions for formation of best REE deposits?

I want to know more about REE deposits.

03 August 2024 6,930 2 View

Why cycling of matter important to life on Earth & movement of matter through an ecosystem different than movement of energy through an ecosystem?

Why is the cycling of matter important to life on Earth and movement of matter through an ecosystem different than the movement of energy through an ecosystem?

03 August 2024 5,061 3 View

Why does the flow of energy through the biosphere depend on the cycling of nutrients and matter and energy flow across the earth's subsystem?

03 August 2024 6,412 0 View

Does atmosphere affect flow of matter & energy on Earth & flow of energy in biosphere related to the flow of food through a food chain?

How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?

02 August 2024 9,644 0 View

How do energy flow and matter change in Earth's interior and geosphere subsystem affect the flow of matter and energy on Earth?

02 August 2024 9,144 0 View

How do matter and energy flow during the interaction of Earth's subsystems and hydrosphere subsystem affect the flow of matter and energy?

02 August 2024 7,579 2 View

How does energy flow through Earth's systems and how does that affect climate and biosphere affect the flow of matter and energy on Earth?

02 August 2024 2,699 2 View

How Do Project Data Analytics and AI Advance Quality 4.0 in Construction Project Management?

As the construction industry advances, the integration of Project Data Analytics and Artificial Intelligence (AI) is becoming increasingly crucial in project management. These technologies are...

31 July 2024 6,484 1 View

What is the best conditions for formation of REE ore deposits?

I want to know more about REE deposits.

31 July 2024 7,366 1 View

If the emission of all greenhouse gases is stopped now, will the temperature of the earth continue to rise?

Here's a serious question: If we were to stop emitting all greenhouse gases right now, would the Earth's temperature start to cool down, or would the existing greenhouse gases continue to warm the...

30 July 2024 5,123 4 View