I recently purchased a vial of Cynom-K1 cells. I grew them up over several weeks as they were of VERY poor viability when they showed up. Expanded them, froze back 35 vials, saved a couple million and did a DNA extraction. Amplified an intron from a region for which I had Cyno and Rhesus sequence to confirm that these were actually Cyno cells. To my surprise they were HUMAN. A warning to anyone getting in cells, make sure you know what you have before starting any work with them.
Haha. Good thing you checked. Your boss should pay you a bonus for not wasting years working with the wrong cells. Happens all the time.
John Schloendorn I can't really take credit for thinking to check. A few years ago we had a project that required a particular cell line from DOGS. Researcher got them in, grew them up and started working with them and nothing worked as it should, he spent 4 months being frustrated then checked the DNA sequence to find that they were from a COW. I simply took that lesson and learned from it!
What's this underselling yourself about? Taking a lesson and learning from it. That's the best you can ever do. I think that's exactly what you get full credit for.
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