Basically i want to trap the cells in the hydrogel. But i'm facing certain problems:
1. Gels are getting detached from the well plate
2. Some of them are dissolving when i add the media to the cells
Please suggest to overcome this problem?
Also i would like to know the seeding density of the cells suspension in gel and how to maintain them?
Thanks
Nisha
In the case of dissolved gel in media, it may be noted that your method to the design of hydrogels is not true. In fact, if you prepared the hydrogels through electrostatic complexing method, (for example alginate-Ca2+), the stability of hydrogels are affected by ionic strength. If the cell cultures are mainly carried out in PBS, the hydrogels will not stable and easily will be dissolved in media.
All hydrogels are differents. To resolve your problem, you have to know its chemical composition and its physical properties.
Some hydrogels are floating. Are you sure your hydrogel has to stay attached ?
Dissolving can be a problem of design or depending on physical properties of your gel.
Hi Nisha,
If you are using collagen hydrogel, use a glass bottom petriplate. The boundary of the plate will reduce the amount of shear your gel will face while pouring the media or PBS.
@ NIsha,
As suggested try making them on glass bottom petriplate. This helped me in making the collagen gel without breaking.
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