Additional bands on Western blot after protein overexpression? What are the additional products?

Dear Researchers, I cloned the IRF9 coding sequence into plasmid LV073. I used the Gibson assebly (NEBuilder) protocol. I cut the vector in MCS with the enzyme ScaI (blunt ends), amplified my...

26 November 2020 5,080 4 View

Has anyone pooled libraries from ChIP and RNA experiments in one lane for sequencing?

I have one lane and samples from two experiments (RNA and ChIP). Is there an option to sequence them together? I am afraid that RNA samples will "eat" all the reads. Thank you for any suggestion!

21 July 2020 1,846 4 View

Is any requirement to the compressed air clearness to use with the laser optics ?

I understand that the compressed air for the laser optics purge (first step of cleaning) such as mirrors or lenses should be "clean", but does anybody can explain me what really does it mean...

01 April 2019 8,021 5 View

Can anybody recommend me a company to buy graphite target useful for PLD technique?

I would like to buy graphite target for further DLC films creation by PLD technique, does anybody have some good experience with company offering it in terms of reliability and good...

14 March 2019 8,314 3 View

Source of DiFi cell line

Do you know where can I buy DiFi cell line? There are not available in ATCC, ECACC or DSMZ.

20 July 2017 3,772 1 View

Can I measure expression of MIR genes on Illumina BeadChip if I prepare cRNA using Ambion Total Prep Kit (based on oligodT primers)?

Because list of probes on the BeadChip contains some of MIR genes (i.e. MIR1-2, MIR1200, MIR1202, MIR1203 etc.) however, as I remember correctly - the mature microRNAs don't contain the polyA...

19 June 2016 2,867 3 View

Has anyone investigated the influence of the head coil type on resting state data?

What do you think about using 8-channel head array coil?

04 January 2014 8,779 2 View

Is there any GC-ECD in NUIG that can be adapt to measure N2O in a gas phase?

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26 November 2013 2,205 1 View

Role of Clostridium genus in gut brain axis. Investigation of neural connectivity loss in Autism Spectrum Disorder (ASD), nosology, treatment and ther

Dear fellows, I am currently working around specified subject. As part of my investigation I recruit volunteers, able to provide faecal tests results, suitable for ASD patients and control....

01 January 1970 6,630 3 View

Drama on the stage vs. new philology

I'm analysing the relation between the work with literary drama on the stage in the theatre and the theory of new philology (medieval texts). Do you have an example for it in the contemporary...

01 January 1970 8,329 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

How to run raise regression in R?

if anybody know how to run raise regression in r, and raise parameter selection, please help me

24 February 2021 8,199 1 View

Can anyone suggest me the best way for Regression analysis of Accidents?

Dear Altruist, I want to make a regression analysis based on accident data. But I am not finding the necessary information about what type of regression is more applicable for analysis accident...

23 February 2021 9,956 3 View