Cryopreservation of freshly dissociated human skin squamous cell carcinoma cells?

08 January 2020 1 8K Report

I am in a situation where I need to freeze down freshly dissociated primary human squamous cell carcinoma cells for later use. Does anyone have any reccomendations on what media/solutions I can use for freezing to maximise cell viability and minimise cell loss when I thaw them out in the future?

Mohammad Tariq Ali Khan

1% sterile dmso along with 10% fbs . prepare media prior storage and keep it in minus 20 degree.

note: add 1mL per cryopreservation vials.

for 10mL, add 1 ml sterile dmso with 9mL FBS,, filter and store.

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