CRISPR-CAS9 conditional knockout mouse

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Can we mark 'EFL Learners shifting from general digital to AI technologies' as technological transition?

After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...

08 August 2024 8,964 4 View

How to generate a citation of my paper from ResearchGate?

How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...

08 August 2024 6,690 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to fix background error in rietveld refinement of one XRD peak using GSAS-II?

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05 August 2024 5,291 2 View

How can I add own Henry coefficients in Aspen Plus?

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05 August 2024 2,333 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

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Can usage of AI tools like chat GPT in research work is recommendable ?

AI tools like ChatGPT can enhance research work significantly when used responsibly and in conjunction with thorough human oversight.

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Usage of internal standards in LC-MS/MS analysis?

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ANY free software for reconstructing neurons in the microscopic image?

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04 August 2024 4,725 2 View

How effective is the Citi Bloc standard basket in enhancing the accuracy and comparability of international construction cost assessments?

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Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

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09 August 2024 9,621 0 View

Could it be a cell culture contamination?

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

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How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

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Why activated CAR-Jurkat cell could not kill targets?

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Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

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"A Markov-like Model for Patient Progression"?

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04 August 2024 4,130 4 View

How to isolate lymphocytes from mouse spleen?

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How to Add Missing Water Molecules in Protein-Membrane Simulations?

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Mahdi Moradpour

Hi Hani,

Through the literature, I found that "ToolGen" kits are more reliable! Although, I work on plants! I think you may like to check below link out to get some ideas:

http://www.toolgen.com/sub03/sub02_02.html

Good luck,

Manizheh Narimani

I thought that Genecopoeia with following link is great.

http://www.genecopoeia.com/

Weiping Yuan

I would suggest you to let the company or university mouse core facility to do the job. You just need to tell them what gene you want to knockout.