Hani Harb

8 Questions 11 Answers 0 Followers

Questions related from Hani Harb

Ear Hair cells cell-line?

Hi Everyone, i am looking for cell lines for ear hair cells from humans. is there anything out there. It is not my field so i have no idea. I appreciate the help.

12 September 2023 9,396 0 View

N-methylhemeanthidine chloride?

Hi Everyone, I am looking for N-methylhemeanthidine chloride for an experiment i am conducting. Does anyone know where i can find it? or someone can extract it for me from Z. candida?

17 September 2019 4,870 0 View

Micro ChIP! I want to know about a good protocol for microChIP for TF-ChIPing?

Hi Everyone, I need to ChIP a Transcription Factor and then do a sequencing. The problem is that my cells are very low and i can't have more than 15.000 cells per animal. Stacking animals may take...

12 November 2018 3,104 0 View

CRISPR-CAS9 conditional knockout mouse

Hi, i want to make a conditional knockout mouse and i am looking for the best kits for that. Can you recommend some kits please

06 July 2017 2,573 3 View

Epigenetic Factors and how do they bind and exert their effect?

Hi everyone, i am looking for some papers explaining how certain epigenetic facotrs are recruited for acetylation or methylation. I mean how does this takes place and why does it affect H3K4 for...

28 March 2016 6,911 1 View

How can I optimise RNA isolation from Rat hippocampus?

I am trying to isolate RNA from rats hippocampus and cortex. Somehow, i am not able to get more than 10 ng/µl. Is that normal? I am using Qiagen kit and it works for all different types of mouse...

20 October 2015 6,930 6 View

DNA missing after ChIP?

Hi everyone, I have done a ChIP for STAT1 transcription factor and I saw a shift in the melting curve. After sequencing, I saw that a 15 nucleotide piece is missing. This was the IRF binding site!...

30 October 2014 9,733 3 View

Staining of Ultrafine particles with a chromophore?

Hi everyone, I would like to stain ultrafine particles from diesel exhaust with a chromophore to trace them. Can anyone advise in this matter please?

01 January 1970 3,938 2 View