Create a consensus sequence using R and F sanger sequences?

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Ajay Saini

Hi,

You may use the forward sequence and the reverse complement of the reverse sequence in any alignment program and generate the consensus sequence

Paul Rutland

Mega software at https://www.megasoftware.net/ is worth a look

Rajeev Meora

You can use CAP3 Assembly Program (https://doua.prabi.fr/software/cap3). It's easy to use. You can find publication information here (10.1101/gr.9.9.868).