Rohan Samir Kumar Sachan

9 Questions 5 Answers 0 Followers

Questions related from Rohan Samir Kumar Sachan

How do I insert recombinant plasmid into algal cells?

I have synthesized recombinant plasmid with my gene of interest. I need to insert the recombinant plasmid into algal cells. Please suggest experimentation literature for the same.

01 December 2023 9,435 4 View

I need to stain bacterial cells with Nile red for fluorescence microscopy examination. Can I perform following protocol?

1. Prepare minimal media for PHA-producing bacterial cells and add 10 microgram/mL DMSO in the media. 2. Inoculate a loopful of culture in the media and incubate for 48-72 hours. 3. After the...

07 July 2023 9,346 0 View

How can I locate specific gene sequence in whole genome, If I have the sequence of that specific gene?

I have the whole genome of a particular bacteria and I have the pha gene sequence of other bacteria of the same group. I want to analyze how much percentage of pha gene is present in the whole...

26 April 2023 9,766 1 View

How to perform methanolysis of PHA for GC-MS?

I am trying to prepare PHA samples for the GC-MS analysis, during the procedure PHA is mixed with an equal volume of acidified methanol and chloroform. The latter is boiled at 100 degrees Celsius...

11 April 2023 3,225 2 View

Why I am facing difficulty in growth of Bacillus spp. for PHA production using pretreated extract of LCW?

I am using an extract of pretreated lignocellulose at differing percentages (100, 75, 50, and 25%) as a carbon source replacing glucose in minimal media (normal composition is glucose, 10g/L;...

02 February 2023 9,010 0 View

Create a consensus sequence using R and F sanger sequences?

Hello, If there is any tool available for generating consensus sequences from R and F sanger sequences? If yes, I would be grateful if anyone could help with this.

28 December 2022 9,939 3 View

How much sugar extract to be used during minimal base agar medium?

I have pretreated the waste and sugar has been liberated in the extract which was confirmed by qualitative Molisch's test and quantitative DNS test. However, how much sugar extract is to be added...

19 January 2022 1,844 0 View

Can someone share standard protocol for DNS test of glucose?

I need to make a standard curve for glucose and find out the concentration of unknown sugar. But I'm unable to get a standard protocol for the same.

20 November 2021 2,910 4 View

How to pretreat wheat straw using alkali for saccharification without enzymatic role?

I need to saccharify wheat straw in order to utilize sugars from it as growth media.

13 November 2021 4,629 2 View