Could you please advise me that any suitable techniques for this experiment which will be done shortly?

I am planning to perform the functional study of gene whuch is supplied maternally, so morpholino-mediated knockdown assay won't work. Also about the CRISPR mediated knockout study need to wait...

27 December 2020 731 1 View

How do we apply a bending stiffness on to a wire model in abaqus?

I was trying to mimic the deflection of a cantilever beam in wire on to a model with a portion of the beam replaced by a spring which provides a bending stiffness. I gave a usual spring with...

01 July 2020 6,869 4 View

How to rectify the"DIsplacement Increment for contact is too big" warning in abaqus?

I am currently modelling a joint of a geodesic dome. I previously ran an analysis only with preload of bolts ignoring all contact surfaces of the shank of the bolt and other geometry . This time...

03 June 2019 4,811 5 View

How to generate adsorption isotherms ?

We are doing a project on adsorption of hexavalent chromium using agricultural wastes. I would want to generate adsorption isotherms for our data. Would I be able to know the procedure for the...

24 May 2019 1,655 13 View

Which is the good and required structure that need to be designed to solve current problems in a society?

I am a final year UG student, this semester we need to do design project. But we have learnt only member design in our curriculum, so I am looking for a structure by designing that we can get a...

15 June 2018 1,986 7 View

How can I address the issue of a significant change in the Control group?

A study on effect of a particular yogic maneuver was carried out in our department. It involved two groups, control and intervention group( n = 50 in each). It so happened that, at the end of...

26 April 2015 4,409 3 View

What are the alternate materials for machine bases other than cast Iron ?

Material science are developing to great extent kindly share the information on alternative material for cast iron. I came to know that some of the machine bases are made of polymer concrete,...

04 February 2015 456 13 View

Which is the best element choice for muliti layered FRP material in ANSYS ?

I have to conduct FE analysis of aircraft structure of GFRP material which is of multi layered kindly suggest me how to do modelling of multi layered elements with different material properties in...

18 December 2014 9,482 4 View

What is the equivalent concentration of lactose to induce T7 based recombinant expression instead of IPTG?

Hi Guys, Lactose is a metabolisable sugar molecule and affected by inducer exclusion and catabolite repression. Any experience of using lactose as inducer instead of IPTG to improve recombinant...

10 November 2014 4,662 3 View

How will co efficient of friction change with respect to the change in contact area ?

Does the co efficient of friction depend only on materials in contact? In frictional clamps the co coefficient of friction plays a major role, as co efficient of friction directly depends on the...

09 October 2014 1,469 19 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Which CpGs have a significant effect on gene expression?

It is known that methylated CpG around TSS decreases gene expression. However, It is difficult to know exactly which CpG has a greater impact on gene expression. Are there any databases known...

20 February 2021 5,634 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View