Hello everyone. I am using a bacterium to control a soil-borne pathogenic fungus on pepper.
I collected root, stem and leaf samples at different time points and I want to analyze the activity of POD, SOD, CAT, PAL and PPO. There are many publications online about enzymatic assays with a lot of different methods. Those confuse me since this is the first time I do enzyme assay. Therefore, could anyone share the protocols that you use with me or suggest me some good papers with clear description about the methods? Your help is very valuable to me.
Thank you all in advance!
Hello
I think the methods presented by the former professor have been sufficient. But if you want your work to be more accurate and easy to apply, I would advise you to use kits(for enzymatic activity). This is because it is the most accurate with that working in the lab is very easy, and quickly and without any damage to the user's health. It also holds all the information and steps used. It's really cool.
Good luck to you.
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