Price and experience of use
Recently l was studying EMSA assay, but l didn't find any detailed information about protein sample. we can use two kinds of protein, including crude protein extracts and recombinant proteins. but...
02 February 2021 10,047 3 View
I must put 50 kb plasmid into ecoli strain. I think 50 kb is too big for heat shock with ecoli. Is that correct? Will electroporation work? Or do I need to use cos sites and lambda phage...
22 January 2021 8,612 3 View
I received several strains of bacteria from another lab far away. However, one of the samples appears to have high fungal contamination. When I grow it in broth, I see lots of fungal...
03 January 2021 2,749 16 View
I made a new bacterial strain with an unmarked deletion using 2 step homologous recombination (with sacB counterselection). The full protocol is given in the link at the bottom. The protocol...
01 January 2021 1,580 4 View
Sometimes my western blots have very uniform/even background, other times they're very uneven and patchy like in the attached image. Why is this? I thought my blots were drying out during...
05 December 2020 8,656 5 View
In the past, I've always used Licor's old blocking buffer that included sodium azide. I would dilute my antibodies in the buffer, stain, re-collect the dilution, and re-use it a few times. The...
28 November 2020 5,795 3 View
I have a low copy plasmid (ori=pBR322) and I wanted change it to high copy (like ori=pUC). This paper seemed to say that only one mutation was required to increase the copy number: High copy...
13 November 2020 7,292 4 View
For western blots, I normally stain with 10 mL buffer with 1:1,000 dilution of my specific antibody. Recently, there is an equipment change in my lab and 10 mL isn't enough volume to cover the...
27 October 2020 2,749 7 View
Why the pink color will change to yellow, when the DMEM media is frozen? however after dissolution in room temperature, media color will change back into pink.
26 October 2020 708 2 View
I must concentrate and purify a lot of dirty lysate and secreted protein samples. I just want to run them on protein gels for western blots. I'm trying to decide the best method of...
10 September 2020 2,911 8 View
Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?
02 March 2021 9,706 5 View
Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...
25 February 2021 3,612 3 View
08 February 2021 7,901 6 View
17 January 2021 301 2 View
Hello I writing a SCI paper and want to make a table for chemical price comparison. However, the chemical price varies from country and company, amount and also dates. Is there any reference...
12 January 2021 5,600 1 View
I am trying to solve a multi-objective optimization problem using the Pyomo library in Python. The objective function includes operational optimization of an electrolyzer for hydrogen production...
11 January 2021 8,018 3 View
hello every one i want to practice deep learning as descibed for a home price prediction problem i use code as following # IMPORTING PACKAGES import pandas as pd # data processing import numpy...
07 January 2021 8,761 1 View
I have stock price data(Open, high, low, close), I want to do prediction by combining two different models like CNN with others. Dataset will be same, what are the ways that I can use for...
04 January 2021 1,845 3 View
Hello ! I have to intern in a lab for 5 months next march and as I want to eventually do research in marine ecology, i want to intern in the same field. I have found two internships, one in...
08 December 2020 900 4 View
It should not be confused with strike and limit thresholds. In this case, I am emphasizing the basis of the index, such as: -Cumulative, accumulated, deficit and excess rainfall values. -Average,...
29 November 2020 3,084 3 View
