Could you offer me protocols for extracting antimicrobial biomolecules from bacteria? Thank you?
Hi!
Well, extracting antimicrobial biomolecules from bacteria is a real challenge. The most common methodologies used are solvent extraction, preparation of free-cells supernatants from liquid cultures, among others. Some people have also used inactivated bacterial cultures by heat, and test the antimicrobial activity.
Do you know which bacterial genus are you working on? So you could know what kind of antimicrobials are produced by your strain (or at least the reported)... then, you could have an idea about their nature and start planning the extraction.
If one is extracting an antimicrobial protein of interest, it might be helpful to run an electrophoresis set up for protein separation rather than DNA sep
Dear Akermi moulay m'hammed ,
To answer your question, you have to clarify which antimicrobial biomolecules, protein or nucleic acids do you want to extract?
For nucleic acid, you can read my article Viet Thanh Nguyen
Article Phytochemicals: Extraction, Isolation, and Identification of...
@ Viet Thanh Nguyen thank you for answering my question; I work on actinomycetes is I am looking for effective antibiotics.
@ Guenaia Abdelkader thank you for answering my question; I work on actinomycetes is I am looking for effective antibiotics.
I recommend you to read the following article: antibacterial activity of actinomycetes isolated from different soil samples of Sheopur (A city of central India). I'm leaving the link below:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3696223/#__ffn_sectitle
- Badges
- Science topic
- Similar topics
- Chemistry
- Biochemistry
- Biomolecules