If we couldn't use our complete cell culture media for long time (1 month). Could we store it in -20 °C?
Hi.
To my experience, freezing cell culture media might cause some precipitations. We are not too sure what the precipitants are, but google results indicate that they may be some insoluble salt complexes. Besides, repeated freeze/thaw cycles may, to a certain extent, degrade proteins (amino acids, L-glutamine, growth factors, etc.)... So the best thing would be to prepare only the amount that you will use within a month or two...
However, sometimes we would use the media which was freeze-thawed only one cycle, onto not-too-sensitive commercialized cell lines (not primary cells), such as HeLa and CHO... There was once i used a media which was prepared and froze 6 months ago, the cell growths were still considered as satisfactory with no obvious sign of cytotoxicity, etc.
Hope it helps :) Take care.
Hi.
To my experience, freezing cell culture media might cause some precipitations. We are not too sure what the precipitants are, but google results indicate that they may be some insoluble salt complexes. Besides, repeated freeze/thaw cycles may, to a certain extent, degrade proteins (amino acids, L-glutamine, growth factors, etc.)... So the best thing would be to prepare only the amount that you will use within a month or two...
However, sometimes we would use the media which was freeze-thawed only one cycle, onto not-too-sensitive commercialized cell lines (not primary cells), such as HeLa and CHO... There was once i used a media which was prepared and froze 6 months ago, the cell growths were still considered as satisfactory with no obvious sign of cytotoxicity, etc.
Hope it helps :) Take care.
Hi Farhad,
one time freezing is okay. the same as you freeze heat inactivated serum at -20. but no freezing-thawing cycles. better store in small aliquots of say 50ml.
best,
Gurjot
Yes you can freeze but as Gurjot Kaur says, store in aliquots, no freeaze thawing cycles. I have done this for growing D3 embryonic mouse stem cells.
Thanks for answering my question so promptly , i'll do it tomorrow.
Best wishes
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