This is our first time handling this Caco-2 cell line. We thawed the vial that we had, and the cells had been growing slowly. There was a week when there was confusion with the medium used, and they were around 5 days without any supplements at all and no antibiotics. After those days, when we analyzed the cells there were these small fragments around in the culture, could this be yeast contamination or could it be cell debris? And is there a solution to this problem?
These are not happy CaCO-2 cells.
As such they cannot provide you with what you seek. It is a false economy to save them; they are no longer related to your master stock sample. Discard and recover a fresh vial.
Hello Your Caco-2 cells do not have a good aspect. There is probably a mistake with you frozing protocol. Froze cells with 30% serum + 10% glycerol, 2 millions of cells/1ml. These cells do not like DMSO.
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