In the case of yeast transformants
The plasmid DNA was shown to organized in nucleosomes and chromatin structure is similar to chromosomal DNA (Nelson and Flagman, 1979).
Hello, I am currently writing my master thesis where I have a 2x2 factorial design with one downstream variable (2 independent and 2 dependent variables). I need to conduct a multivariate...
11 February 2021 6,014 4 View
Hi, help please, how to cancel the authorship of the article Civilizational Phenomenon of Narcissism: Vectors of Objectification in a Paradigm of Psychoanalysis ?
15 December 2019 9,043 3 View
I would greatly appreciate for help in the suggestion of company for ITS1 and IT4 primers purchasing in Europe. Thank you.
12 September 2017 469 3 View
Dear colleagues, I need to estimate the basic soil characteristics - total carbon, nitrogen, potassium content. I also need the content of Fe and halogens (F, Cl, maybe I). The point is that I can...
12 February 2015 4,874 9 View
Phylogenetic analysis (combining molecular and morphological data).
10 August 2014 4,406 7 View
Zou Finrock and Kelly Summers have recommended me to contact you. We are using 8-day old Arabidopsis plants to see of the effects of toxic metals, in the root tips, in particular. They are really...
01 January 1970 5,600 3 View
We understand that in sharing economy, access to usage will be important. How will the attitude to ownership and disposal of property change? Do you want be the owners?
01 January 1970 546 8 View
Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...
01 March 2021 210 1 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...
28 February 2021 5,440 3 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...
28 February 2021 4,868 2 View
Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?
28 February 2021 8,095 1 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...
25 February 2021 1,011 3 View
After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...
25 February 2021 5,712 3 View