In the case of yeast transformants
The plasmid DNA was shown to organized in nucleosomes and chromatin structure is similar to chromosomal DNA (Nelson and Flagman, 1979).
I need to publish an article in an ornithological journal.
20 July 2024 862 5 View
What programs, websites do you use when building maps?
31 January 2024 6,388 7 View
Please help me find free ornithological journals for publishing articles that describe regional bird studies?
31 January 2024 8,536 3 View
Please share the seminars or conferences in biology, zoology, ornithology that will be held in 2024? Is it free to join them?
03 January 2024 6,010 6 View
We currently use clustermarket to manage booking of our instruments, but they decided to start paid subscriptions very soon. I would be really grateful for any advise for decent online booking...
10 October 2023 6,411 0 View
What are the free journals on acarology?
11 June 2023 7,757 3 View
What are the key challenges and opportunities for fostering sustainable entrepreneurship and innovation in Ukraine, and how can they be effectively addressed to promote sustainable economic growth?
17 March 2023 5,200 1 View
I am planning an MDA-MB-231 xenograft experiment for a drug efficiency study. Based on the literature review, I am planning to inject 0.8x106 cells in 100ul PBS into the 4th mammary gland of BalbC...
07 February 2023 6,500 4 View
Need advice: Patient 37 y.o. with DVT and thrombosis of right radial artery (after coronary angio 2 weeks ago), pulmonary embolism and thrombolysis month ago. Now patient has indication for...
17 September 2022 5,132 5 View
A peer reviewer needed.
24 October 2021 2,198 4 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...
05 August 2024 1,573 4 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I am conducting my analysis using SPSS. I log transformed my data using In(X+1) as my data contain zero values. However, when I want to back transform the regression coefficients generated from my...
31 July 2024 7,860 3 View
Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...
30 July 2024 6,648 1 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View