What are the research areas that combine quantum computing and HCI?

I starting studying human-computer interaction, and its aim to provide effective interaction between human and computer. Because of its ubiquitous nature, I am looking for research topics, present...

06 December 2020 1,235 2 View

How to computationally study the effect of solvent in slurry phase polymerization?

I want to study the effect of the n-hexane solvent in the Ziegler-Natta polymerization reaction. Since the reaction is performed in the slurry phase, I guess there is no need to use the COSMO...

19 August 2020 6,260 7 View

How can we visualize the transport of groundwater ions at study area without using MODFLOW MT3DMS model?

There is so much requirement of data for modeling the flow of ions in MODFLOW MT3DMS which may complicate the study. If you suggest some more method/document to do or a proper way of the use of...

17 April 2020 5,033 3 View

What are the thermal conductivity values of various metal oxide particles such as Aluminium oxide and Copper oxide?

03 October 2016 9,327 10 View

Identification of fungi?

i have isolated fungus from plants i am attaching microscopic pictures and plate and i want to know the name of fungus.

26 September 2016 1,357 17 View

What is the best procedure of antimicrobial activity?

i have methonolic extract of fungus and i want to do antimicrobial activity. what is the best procedure to follow disc diffusion or agar well method or in what quantity extract is use.

08 August 2016 9,258 13 View

How to calculate total phenolics and flavonoid content?

i want to calculate flavonoid and phenolic content of my crude fungus extract in mg/g of dry mass), is their any easiest way to calculate. mycelia dry weight = 2.40 gm total extract 237 mg

08 August 2016 1,343 3 View

How to incorporate thermophysical property variation with temperature for an internal forced convection scenario through a tube at constant heat flux?

Bulk mean temperature of the fluid is generally calculated by taking the average of inlet and exit temperatures of the fluid for constant wall temperature boundary condition.but in case of...

02 July 2016 9,234 6 View

Relaxation in metal using vasp?

12 June 2016 3,786 3 View

In a typical heat exchanger, is there any specific rule that the hot fluid should flow through the inner tubes??

In any heat exchanger, for example; in a double pipe heat exchanger,how do we decide whether the hot fluid should flow through inner tube or outer tube?. Or is it completely irrelevant whether the...

18 February 2016 4,576 18 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Is it possible to quantify recombinant protein in the supernatant using SDS PAGE ?

I would like to quantify a recombinant protein (with native signal sequence) secreted by Pichia pastoris using the SDS PAGE. Since my standard protein (plant origin) is not pure, I would like to...

07 February 2021 3,880 9 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to prepare protein samples in EMSA assay?

Recently l was studying EMSA assay, but l didn't find any detailed information about protein sample. we can use two kinds of protein, including crude protein extracts and recombinant proteins. but...

02 February 2021 10,047 3 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

Alpha synuclein lack structurw (i.e some of the helix part goes into turn ), any cause behind this?

I am trying to simulate alpha synuclein (1xq8.pdb) in GROMACS using GROMOS53a6 ff. I have run it for 200 ns. At the end of simulation alpha helix protein losses its helical structure. I am not...

31 January 2021 4,285 3 View

How to increase the lower detection limit of ELISA?

Hi all, I am trying to establish an ELISA method for the quantification of a recombinant protein injected into mice. In the meantime I am trying to make calibration curves with the purified...

27 January 2021 8,702 3 View