Soon I will try genotyping based on rRNA using isothermal amplification and I want to learn if anyone can suggest a simple and fast isolation method for rRNA or totalRNA. I dont need to achieve high purity.
Subhojit Sen
One can use density gradient isopycnic centrifugation to purify ribosomes as ribonuceloprotein (RNP) particles and then extract RNA from them. This was how the swedberg coefficient for the ribosomes was calculated and can now be used to purify them by setting up appropriate density gradients for separation of these relatively large RNPs away from other smaller RNPs.
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