Im interested to see the functional analysis of CYP1B1 gene in humans, but confused with how to get cDNA of whole CYP1B1 gene after its amplification in different fragments in various PCRs?
Current protocols in molecular biology is a good start. They give you great background, methods and great overview of troubleshooting. Very helpful
http://onlinelibrary.wiley.com/doi/10.1002/0471250953.bi0401s6/full
Thanks for your support
Stratagene protocol is the best to understand it
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