Salam
May be this paper can help you
http://www.tandfonline.com.sci-hub.org/doi/abs/10.1080/10826076.2010.534399
Take a look on our study. I hope it helps.
Contribution to the Study of Phytosterols and Phenolics from the Barks and Leaves of the Trichilia Catigua A. Juss., Meliaceae
salam
In order to confirm the identity of the phenolic compounds that could not be done by HPLC–UV, additional analysis was carried out using HPLC with mass spectrometry detection equipped with an API source and employing an ESI (electrospray ionization) interface. . Solvents used were ultrapure water (A), HPLC-grade acetonitrile (B), formic acid 1% (C) and formic acid 1% HPLC-grade cetonitrile (90:10) (D) at a flow rate of 1 mL min1. Elution program start with 100% C, the gradient was the following: from 100% C to 100% D in 3 min, from 100% D to 1% B in 4 min, isocratically 1% B in 3 min, from 1% B to 12% B in 20 min, from 12% B to 50% B in 5 min, isocratically 50% B in 5 min, from 50% B to 100% C in 2 min . Spectra were recorded in the positive ion mode and the MS detector was programmed to perform a series of consecutive scan: full scan from m/z 150 to 1500. (Mraihi et al., 2014). http://dx.doi.org/10.1016/j.arabjc.2014.11.045
Dear Mraihi Farouk
infact, i don't have LC-MS,but only UV-VIS and C18 column
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