What is known about the mechanisms of CAR/NR1I3 activation via bilirubin in human hepatocytes?

Thank you for any input!

06 July 2016 3,737 2 View

What is the time scale for transcription-translation events following overexpression via transfection with cDNA vector?

Does it make sense to analyse the mRNA expression of a downstream target gene of an overexpressed transcription factor 4 hours after transfection of a TF cDNA (the cells do not express this TF...

05 June 2014 296 1 View

Apoptin is a potent inducer of apoptosis specifically in cancer cells - why is it still not used in the clinics?

02 March 2014 4,655 24 View

How to prove functional activity of the TF-binding site within an intron identified via ChIP-Seq?

31 December 2013 5,432 28 View

Your Top 5 non-fiction books related to science

I am searching for fancy books which could be of interest for broad biological community. Something like real stories behind great discoveries, biographical anecdotes from great scientist, etc....

08 September 2013 1,484 5 View

Tissue-specific gene expression: how to "read" datasets?

To get the first hint about tissue distribution of a particular protein I usually refer to GeneCards. There you can find at least three different datasets from BioGPS, RNASeq (Illumina Body Map)...

05 June 2013 9,623 9 View

Influence of microRNA on alternative splicing

I currently study one protein, which is represented in two isoforms in human cells: wild-type transcript giving rise to the full length functional protein and truncated variant, lacking the entire...

02 March 2013 5,895 4 View

Survival curves for stable clone generation

Does anybody know a resource for the concentrations of different antibiotics (like Hygromycin, Blasticidin, G418 etc) which should be used for the generation of stable clones in different cell...

01 February 2013 1,679 10 View

Editorial board of an online journal.

I would like to become an editor of a board of some online journal, but I'm not really familiar with the process of hiring the editors. Could I send an application for example with an...

01 February 2013 7,740 5 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View