Could someone share his/her experience in using SOMAmers (Slow Off-rate Modified Aptamer) reagents in detecting the same protein using Aptamers or antibodies?
Thank you!
Thank you for any input!
06 July 2016 3,880 2 View
Does it make sense to analyse the mRNA expression of a downstream target gene of an overexpressed transcription factor 4 hours after transfection of a TF cDNA (the cells do not express this TF...
05 June 2014 409 1 View
Since the first report in 1997 it is known that the chicken anemia virus protein, apoptin, induces apoptosis specifically in human transformed and malignant cells but not in normal cells. There...
02 March 2014 4,780 24 View
We´ve got data strongly indicating that TF regulates the gene of interest via direct binding within an intron and not in the promoter region. How can I prove the functional activity of such a...
31 December 2013 5,844 28 View
I am searching for fancy books which could be of interest for broad biological community. Something like real stories behind great discoveries, biographical anecdotes from great scientist, etc....
08 September 2013 1,597 5 View
To get the first hint about tissue distribution of a particular protein I usually refer to GeneCards. There you can find at least three different datasets from BioGPS, RNASeq (Illumina Body Map)...
05 June 2013 9,750 9 View
I currently study one protein, which is represented in two isoforms in human cells: wild-type transcript giving rise to the full length functional protein and truncated variant, lacking the entire...
02 March 2013 6,007 4 View
Does anybody know a resource for the concentrations of different antibiotics (like Hygromycin, Blasticidin, G418 etc) which should be used for the generation of stable clones in different cell...
01 February 2013 1,842 10 View
I would like to become an editor of a board of some online journal, but I'm not really familiar with the process of hiring the editors. Could I send an application for example with an...
01 February 2013 7,856 5 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,156 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 503 3 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,987 3 View
Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?
06 August 2024 978 5 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,493 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,246 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,726 4 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
Molecular docking software/ websites?
02 August 2024 8,703 7 View