10 Questions 61 Answers 0 Followers
Questions related from Maria Thomas
Thank you for any input!
07 July 2016 3,840 2 View
Could someone share his/her experience in using SOMAmers (Slow Off-rate Modified Aptamer) reagents in detecting the same protein using Aptamers or antibodies? Thank you!
11 November 2015 5,732 0 View
Does it make sense to analyse the mRNA expression of a downstream target gene of an overexpressed transcription factor 4 hours after transfection of a TF cDNA (the cells do not express this TF...
06 June 2014 377 1 View
Since the first report in 1997 it is known that the chicken anemia virus protein, apoptin, induces apoptosis specifically in human transformed and malignant cells but not in normal cells. There...
03 March 2014 4,742 24 View
We´ve got data strongly indicating that TF regulates the gene of interest via direct binding within an intron and not in the promoter region. How can I prove the functional activity of such a...
01 January 2014 5,751 28 View
I am searching for fancy books which could be of interest for broad biological community. Something like real stories behind great discoveries, biographical anecdotes from great scientist, etc....
09 September 2013 1,565 5 View
To get the first hint about tissue distribution of a particular protein I usually refer to GeneCards. There you can find at least three different datasets from BioGPS, RNASeq (Illumina Body Map)...
06 June 2013 9,707 9 View
I currently study one protein, which is represented in two isoforms in human cells: wild-type transcript giving rise to the full length functional protein and truncated variant, lacking the entire...
03 March 2013 5,977 4 View
I would like to become an editor of a board of some online journal, but I'm not really familiar with the process of hiring the editors. Could I send an application for example with an...
02 February 2013 7,820 5 View
Does anybody know a resource for the concentrations of different antibiotics (like Hygromycin, Blasticidin, G418 etc) which should be used for the generation of stable clones in different cell...
02 February 2013 1,794 10 View