Could someone recommend an online program for gene designing and sequence reading?

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How to prepare E. coli for SEM image?

I'm developing some protein surface displaying system that works in E. coli, I need to take SEM images shows successful displaying of some peptides on the surface of E. coli. However I don't...

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How can I control the expression and solubility of fused protein?

I'm screening a random mutated protein to improve its expression and solubility level in E. coli. So my idea of screening is, fusing it up-stream of RFP or GFP CDS, and by detecting the intensity...

31 December 2017 3,842 4 View

How can I kill E. coli and keep its protein?

I'm trying to synthesize protein outside the bacterial cell wall. I would check its function in a dead E. coli bodies. So I'm trying to find the best way to kill E. coli without affecting this...

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Can pET-15b vector expressed its insertion into DH5a competent cells ?

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07 August 2016 2,490 2 View

How can estimate quantity or quality of proteins that secreted outside E. coli ?

I'm using his tag to measure the type and amount of proteins that secreted outside E. coli cells through Auto-transporter (surface display system). How I can prepare my sample for gel...

31 December 2015 3,144 3 View

How can I measure the gene expression or gene activity?

Hello every one, I have been inserted Metallothionein gene and lead binding poly peptide into SB1C3 vector and transformed resulted plasmid in E. coli DH5a. How I can measure the gene expression...

01 February 2015 8,373 6 View

What is the best way to measure DNA concentration depending on gel electrophoresis? Any supporter programs?

Via agarose gel picture

02 March 2014 7,438 11 View

I'm searching for some useful bioinformatics courses?

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01 January 1970 6,522 5 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

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11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

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GC-MS retention index prediticon?

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I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

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Does crude extraction using NaOH and Tris work well with Fungi?

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Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

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Weak DAPI staining after immunohistochemistry - how to improve?

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Why after performing site directed mutagenesis ,I don't see any colony after transformation?

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Anyone having idea about VN primer for miRNA primer design ?

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Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

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John Schloendorn

Addgene Analyze is excellent.

Kai Ove Skaftnesmo

I recomend j5 ( https://j5.jbei.org/index.php/Main_Page) everyvector (http://www.everyvector.com/users/login)

Samy El-Zaeem

Uriel Barboza Perez

tinkercell :) we used it to make some of our igem circuits.. it can also be used to make plasmids.. but for the plasmids we used geneious

Rodrigo Pereira

You can use RBSFinder

ftp://ftp.tigr.org/pub/software/RBSfinder/

And Plasmapper

http://wishart.biology.ualberta.ca/PlasMapper/