9 Questions 22 Answers 0 Followers
Questions related from Mohamed Shehata
I'm developing some protein surface displaying system that works in E. coli, I need to take SEM images shows successful displaying of some peptides on the surface of E. coli. However I don't...
03 March 2019 9,606 1 View
I'm screening a random mutated protein to improve its expression and solubility level in E. coli. So my idea of screening is, fusing it up-stream of RFP or GFP CDS, and by detecting the intensity...
01 January 2018 3,797 4 View
I'm trying to synthesize protein outside the bacterial cell wall. I would check its function in a dead E. coli bodies. So I'm trying to find the best way to kill E. coli without affecting this...
04 April 2017 8,292 2 View
I have inserted my protein followed by RFP into pET 15-b vector. but I didn't notice any red color in LB culture in presence and absence of IPTG. So, Should I transform the correct plasmid into...
08 August 2016 2,456 2 View
I'm using his tag to measure the type and amount of proteins that secreted outside E. coli cells through Auto-transporter (surface display system). How I can prepare my sample for gel...
01 January 2016 3,111 3 View
Hello every one, I have been inserted Metallothionein gene and lead binding poly peptide into SB1C3 vector and transformed resulted plasmid in E. coli DH5a. How I can measure the gene expression...
02 February 2015 8,332 6 View
I did some experiments using bio-brick parts and I want to show my materials and results using simple and easy online or free program which can show the different components of gene (promoter,...
12 December 2014 4,069 5 View
Via agarose gel picture
03 March 2014 7,395 11 View
I have some experience for designing DNA and protein, expressing and editing gens, however I'm feeling that being familiar with some programs or software that can predict the 3 dimensional...
01 January 1970 6,493 5 View
