I did some experiments using bio-brick parts and I want to show my materials and results using simple and easy online or free program which can show the different components of gene (promoter, RBS, CDS, and Terminators.
Addgene Analyze is excellent.
I recomend j5 ( https://j5.jbei.org/index.php/Main_Page) everyvector (http://www.everyvector.com/users/login)
tinkercell :) we used it to make some of our igem circuits.. it can also be used to make plasmids.. but for the plasmids we used geneious
You can use RBSFinder
ftp://ftp.tigr.org/pub/software/RBSfinder/
And Plasmapper
http://wishart.biology.ualberta.ca/PlasMapper/
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