Mohamed Shehata

7 Questions 22 Answers 0 Followers

Questions related from Mohamed Shehata

How to prepare E. coli for SEM image?

I'm developing some protein surface displaying system that works in E. coli, I need to take SEM images shows successful displaying of some peptides on the surface of E. coli. However I don't...

21 March 2019 8,321 1 View

Could someone recommended good kits for Error Prone PCR?

I'm planing to insert random mutations in a protein (100 aa) to check its binding affinity to another short polypeptide. my planing is to insert this random mutations using EP-PCR. I checked the...

06 November 2018 3,721 2 View

How can I kill E. coli and keep its protein?

I'm trying to synthesize protein outside the bacterial cell wall. I would check its function in a dead E. coli bodies. So I'm trying to find the best way to kill E. coli without affecting this...

06 April 2017 3,737 1 View

Can pET-15b vector expressed its insertion into DH5a competent cells ?

I have inserted my protein followed by RFP into pET 15-b vector. but I didn't notice any red color in LB culture in presence and absence of IPTG. So, Should I transform the correct plasmid into...

17 August 2016 419 2 View

Could someone recommend an online program for gene designing and sequence reading?

I did some experiments using bio-brick parts and I want to show my materials and results using simple and easy online or free program which can show the different components of gene (promoter,...

12 December 2014 4,089 5 View

What is a suitable bacterial media or solution which can be used to determine bacterial heavy metal toxicity and accumulation efficiency?

I tried to check Pb toxicity and accumulation efficiency for my recombinant E. coli strain which contains Pb binding protein in metallothionein ligated in pSB1C3 vector. I used LB media but I got...

28 October 2014 7,824 6 View

What is the best way to carry out Nitric Acid digestion?

I want to digest some bacterial samples using Nitric acid to measure heavy metal content (Pb, Cd, Cu, Hg)I found many kinds of nitric acid in chemicals catalog but I don't know what is the best...

30 July 2014 165 18 View