7 Questions 22 Answers 0 Followers
Questions related from Mohamed Shehata
I'm developing some protein surface displaying system that works in E. coli, I need to take SEM images shows successful displaying of some peptides on the surface of E. coli. However I don't...
21 March 2019 8,323 1 View
I'm planing to insert random mutations in a protein (100 aa) to check its binding affinity to another short polypeptide. my planing is to insert this random mutations using EP-PCR. I checked the...
06 November 2018 3,723 2 View
I'm trying to synthesize protein outside the bacterial cell wall. I would check its function in a dead E. coli bodies. So I'm trying to find the best way to kill E. coli without affecting this...
06 April 2017 3,739 1 View
I have inserted my protein followed by RFP into pET 15-b vector. but I didn't notice any red color in LB culture in presence and absence of IPTG. So, Should I transform the correct plasmid into...
17 August 2016 422 2 View
I did some experiments using bio-brick parts and I want to show my materials and results using simple and easy online or free program which can show the different components of gene (promoter,...
12 December 2014 4,093 5 View
I tried to check Pb toxicity and accumulation efficiency for my recombinant E. coli strain which contains Pb binding protein in metallothionein ligated in pSB1C3 vector. I used LB media but I got...
28 October 2014 7,826 6 View
I want to digest some bacterial samples using Nitric acid to measure heavy metal content (Pb, Cd, Cu, Hg)I found many kinds of nitric acid in chemicals catalog but I don't know what is the best...
30 July 2014 166 18 View
