I have collected soil from two types of soil: I got sclerotia of R. solani and then ..I have to bury them in the soil to isolate biocontrol agents. What would be the experimental design, treatments, and applications from the beginning of my research?
Dear Abbas,
You can download some my published papers to assist you in your project.
With my best wishes
Yours
Ammar
https://www.researchgate.net/publication/283582262_Wastewater_Flocculation_Using_a_New_Hybrid_Copolymer_Modeling_and_Optimization_by_Response_Surface_Methodology
AND
https://www.researchgate.net/publication/277446971_Modeling_and_Optimization_of_New_Flocculant_Dosage_and_pH_for_Flocculation_Removal_of_Pollutants_from_Wastewater?ev=prf_pub
Article Wastewater Flocculation Using a New Hybrid Copolymer: Modeli...
Article Modeling and Optimization of New Flocculant Dosage and pH fo...
Hi Abbas
I think you can design the experiments as CRD, and 3 replicate for every treatment
Dear Aqleem Abbas ,
I agree with Dr. Laith Al-Ani
You can design your project as CRD with tow factors ( the fungus and soil ) and replicate the treatments three times .
Best Regards
Dear Aqleem
you can use factorial experiment in CRD design .Factor A the type of soil(Two levels) and factor B the depth of Sclerotia buried in soil 4 levels (10cm,15 cm, 20 cm ,25,cm).
Best Regard
Hello Abbas:
I am agree with colleagues, you can use factorial experiment in CRD design with two factors( factor A type of soil and factor B the depth of Sclerotia buried in soil in 3 or 4 levels, hence you can made 3 or 4 replication per treatment. For example if you choose 4 levels of Sclerotinia depth with 4 replications per trait, you will have 32 samples, if you can put more samples per replication it would be better in order to reduce residuals when you will analyze your data. Don't forget to randomized experiment and considered that is a fixed model.
Best regards
What would be the treatments? How many treatments? Yes Soil type and depth of sclerotia will be factors what about treatments
Treatments: Soil1 Depth1, Soil1Depth2, Soil1Depth3, Soil1Depth4, Soil2Depht1, Soil2Depht2, Soil2Depth3,Soil2Depth4, you can consider 4 replications per treatment, for that reason you have 32 experimental units in the experiment CRD with 2 factors.
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