We have blood contained in paxgene tubes and would like to know whether Trizol method would be effective in isolating RNA. Is there any modification to be made in the trizol method, for isolating RNA from blood contained in Paxgene tubes to obtain maximum yeild and integrity.

Blood samples once collected in paxgene tubes are stable for a long time and are required to be extracted using the paxgene extraction kit. Instead of using the kit, I used trizol method for the same, and got a single faint band(opposed to 3 bands).