Could occult Hepatitis B, increase risk of liver disease fibrosis progression or development of HCC?

More Hailemichael Desalegn Mekonnen's questions See All

How can we make the solid waste-circular economy nexus in the SDG a reality? How can this nexus of sustainability be measured?

What is the circular economy? How can we make the solid waste-circular economy nexus in the SDG a reality? How can this nexus of sustainability be measured?

23 June 2023 5,960 3 View

Do you have any updates about how to export the results of the Google Scholar search to Excel?

I try to use (http://www.harzing.com/pop.htm) to export the results of the Google Scholar search to Excel, but it's not working.

07 June 2023 9,765 0 View

How many latent variables are allowed in AMOS?

Dears, I have 16 latent variables, and I planned to use AMOS. Is it possible to use AMOS or does the model become saturated?

22 April 2023 2,363 7 View

What is the best way to arrange data in excel for R or CMA software to do multiple treatment meta-analyses in animal nutrition?

We have extracted the data for meta-analysis, but we need some examples on how to code data for multiple treatment studies on animal nutrition.

22 January 2023 9,140 2 View

Does anyone knows how can I prepare Sudan black b solution for dual Sudan black B-ZN staining for M. tuberculosis?

Hello everyone, How are you? Is there anyone who can help me how to do a dual Sudan black B (SBB) and ZN staining for M. tuberculosis? Specifically how to prepare the working solution for SBB and...

13 April 2022 2,438 1 View

Could you recommend updated info or discussion groups regarding the use of hairy root cultures to produce tannin or flavonoids from medicinal plants?

An efficient and cost-effective laboratory procedure for obtaining tannin and flavonoids from hairy root cultures

04 April 2022 9,780 2 View

Can you please tell me what are the best references and guidelines to use to analyze raw 16S rRNA gene sequencing data for bacterial diversity?

References or manuals list to analyze 16S rRNA rumen bacteria data

31 March 2022 3,460 2 View

As all you know measring quality of health care a given activity is not easy? How do you measure quality of Postenatal care services?

Since various scholars state different kinds of models and theories in measuring the quality of health care. Which way do you suggest me to use is appropriate to measure the composite level...

16 October 2021 2,387 4 View

Could you suggest me the data type/management ways with examples for Alpha, Beta, and Gamma diversity analysis using R ?

There are different packages (vegan, BiodiversityR, vegetarian etc.) of R studio environment to conduct diversity analysis (Alpha, Beta, Gamma). hence I have interested to process my research data...

08 August 2021 4,483 3 View

In a quantitative research, how many sample size is enough for observation compliance?

I need to do healthcare providers' compliance to the standard performance with observation, but difficult to get a reference for sample size determination.

09 July 2021 531 6 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

Does anyone notice the tumor regression impact of Hepa1-6 cells in HCC orthotopic mice model?

To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...

18 July 2024 9,177 2 View

What will be the best way to test NFkb activation via western blot?

Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?

18 July 2024 5,214 4 View

Why is the baseline fibrosis level of HK-2 cells very high?

In many experiments where HK-2 cells were stimulated with TGF-β at 10 ng/mL for 48 hours, I observed that even in the control group, the baseline expression level of fibrosis proteins like FN1 and...

15 July 2024 1,929 2 View

Validated HPLC method for the estimation of the Amphotericin B ?

During the release test of Amphotericin B using 1% Tween 80, i observed three peaks even in the standard. The first peak consistently yielded results around 500%, while the other peaks showed...

08 July 2024 1,330 0 View

Do you have a protocol for stimulation of mouse B cells by PWM?

Looking for a protocol for the stimulation of B cells with pokeween mitogen

22 June 2024 4,882 4 View

Asking for a vector, containing Hygromycin B resistance gene?

In my work, Hygromycin B resistance gene is require for screening. I tried to amplify this gene from the vector pIJ941 but failed. I will be thankful, if anyone help me out from this problem by...

19 June 2024 7,822 3 View

LPS dose for RAW 264.7 NF-kB macrophage?

I am working on a project that requires stimulating RAW 264.7 NF-kB macrophage and readout my signal by luciferase assay. Although all concentrations tested (10, 50, 100ng/mL) after 24 hours...

16 June 2024 2,885 3 View

Inconsistente results with cell line LX-2, any advice?

Hi there! I'm working on my master's thesis and for my project I'm testing a new curcumin derivative in liver fibrosis. I'm working with the cell line LX-2 and have been having problems since the...

21 May 2024 2,171 0 View

I did a Renilla luciferase assay and the empty wells do have a high absorbance readout. Also there is variation in my triplicates?

I use raw 264.7 NF-kB cell line and the empty wells has a high absorbance as close as the blank well.

20 May 2024 1,518 4 View

Suraj Kapoor

Yes, occult hepatitis B infection (OBI) can accelerate liver fibrosis progression and increase the risk of developing hepatocellular carcinoma (HCC). This risk is pronounced in patients with other chronic liver diseases, including hepatitis C or cirrhosis. OBI maintains pro-oncogenic potential similar to overt HBV infection, contributing to persistent liver inflammation and carcinogenesis

Yassir Hamadalnil

Yes, The DNA of HBV is still there and replicating in low levels, only HBsAg negative, so genetic integration and genome instability were ongoing, predisposing to HCC.

Clinically, a good study done in a cohort base design they found significant association with Occult compared to overt chroni HBV infection , in liver cirrhosis and HCC (Squadrido, 2006).

Thanks prof, interesting