What are the maximum number of items should be in a iif graph plot?

I found it when I put all the items at a time to differentiate the lines

03 December 2020 7,476 3 View

How do we assess information security in a public organisation?

Dear all, I am wondering if anyone can point me to studies discussing non-technical measurement tools to assess information security in a public organisation. I am planning to explore the...

23 November 2020 3,497 6 View

Plant molecular biology ?

I want to add 1 ug or (1000ng ) of Plasmid DNA in a reaction mixture and add water for a final volume of 50 ul. my plasmid DNA a concentration of 68.47 ng/ul in which I have total DNA volume...

22 September 2020 7,325 6 View

How much of mineral element can be served by a portion of food in comparison to recommended daily intake?

The mineral contents were calculated for a certain grains in their raw form. How can we calculate how much a given mineral, say; zinc or iron can be served by a portion of the flour sample per 100...

15 August 2019 4,731 2 View

What is the appropriate stastical software to analysis for scale data collected from infected(inoculated) and control group(non infected) of plants?

I was collected my ten scale level data from from infected and control(non inoculated) by plant pathogen. Therefore, which statistical tools is appropriate for my data analysis?

09 February 2019 6,854 9 View

Constructing the genomic environments surrounding a gene?

Greetings! I am working on E. coli antibiotics resistance genes investigation and had my isolate sequenced (the whole plasmid by using NGS) for locating my gene of interest. I would like to...

03 March 2018 9,059 3 View

What is the general behavior in relation to the correlation between the protein content and the fat content of plant foods, specifically legumes?

There are various arrays of trends and interactions between nutrients in a given food sample. The correlation between some of them is clear, as evidenced from my own result while working on the...

09 October 2017 4,377 4 View

What do the following ratios indicate in phytochemical and antioxidant study?

What is the implication of the ratio of TFC/TPC in phenolics analysis and antioxidant potency composite (APC) index? In quite a large number of articles, the TFC/TPC is estimated to be less than...

20 September 2017 2,234 2 View

Thermodynamics properties of R404A ?

Efficiency Longevity Performance Capacity Inlet and discharge gas temp. Refrigerant propertiest

01 October 2016 6,171 2 View

How can we measure crop growth stages?

i am conducting an experiment to determine crop water requirement and crop coefficient calibration for tomato at different growth stages. So, is there any simple, cost effective and accurate...

19 January 2016 3,934 7 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

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02 March 2021 932 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

No title

19 February 2021 8,647 3 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View