Could IL-10 in spleen play a role in Cryptosporidium spp. infection?
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Pig slurry is rich in major and minor nutrients. Is there any way to improve / Enrich its manure quality to be used in agriculture organically ? please share your knowledge.
09 August 2024 5,605 2 View
Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...
07 August 2024 269 3 View
How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?
07 August 2024 2,132 3 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Dear fellow researchers, I am currently working on a paper where I need to provide a reliable reference that defines and distinguishes between 3D mesh models and 3D city models. Although I am...
06 August 2024 9,986 2 View
Hi How can this equation Ln(LA) = 1.038 + 0.89 ln(X) be applied to calculate the leaf area of a tomato? Can you explain with an example and what is the substitution of Ln and ln?
06 August 2024 2,508 2 View
Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?
06 August 2024 980 5 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...
05 August 2024 9,805 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I am attempting to culture tissue resident T cells (TRM) derived from fresh intestinal tissue for up to six days. I am finding that the viability of the T cells is low at D0, and a very small...
08 July 2024 4,887 2 View
Hello everyone, I am isolating PBMCs and stimulating them using different stimulation conditions. my question is, I want to add IL-2 to the medium for better viability after stimulation, Would you...
16 June 2024 928 1 View
Thank you!
05 June 2024 3,342 2 View
Hi all, I am working on a project which needs expansion of CD45+ selected cells from tumor and lymph nodes. My Tumor samples expand good but my samples from the lymph nodes are less and less...
23 May 2024 2,839 0 View
What percentage of IL-17 positive cells are expected to be positive in Th17-polarized cells in flow cytometry? Day-1: Coating anti-CD3 and anti-CD28 Day 0: Seeding of purified CD4 cells +...
15 May 2024 7,056 0 View
Currently, there are numerous blood biomarkers that have been linked to fractures and increased fracture risk (for example, IL-6). Considering the current evidence, which ones do you think are the...
10 May 2024 6,097 2 View
Hello everyone. I have a question regarding CFSE proliferation assay with mice. My goal is to see CD3, CD4, and CD8 populations. Why do I keep getting one peak, as if there is no proliferation? I...
08 May 2024 7,744 0 View
Any pointers on how to get Cryptosporidium sporozoites to glide on non-cell substrates? We've tried oocysts from a couple of different sources (WaterBourne and the production lab at U of Arizona,...
07 May 2024 4,620 0 View
Hello, I am having inconsistent results from the suppression assay. The brief protocol: 1. With CD4 microbeads, sorted CD4 --> ~98% of purity was checked each time with flow cytometer. 2....
29 April 2024 1,751 0 View
