Could I use O-nitro- phenyl-a-D-glucopyranoside or maltose instead p-nitrophenyl-a-D-glucopyranoside as a substrate in a-glucosidase inhibition by plant extract?
Saif Ali Yes, you can use O-nitrophenyl-α-D-glucopyranoside (ONPG) or maltose instead of p-nitrophenyl-α-D-glucopyranoside (pNPG) as a substrate in α-glucosidase inhibition studies using plant extracts.
In α-glucosidase inhibition assays, the choice of substrate can vary depending on the specific research objectives and the characteristics of the plant extract being tested. All three substrates, ONPG, pNPG, and maltose, are commonly used in these types of experiments to assess the inhibitory activity of α-glucosidase.