Yes, you can store it at -20 C without any problem. In some protocols it is reccomended to not store for more than one month, but actually it can be stored for several months. For longer periods (years) I would prefer to purify, suspend in TE and store at -20 or even better at -80C.
Yes, you can store it at -20 C without any problem. In some protocols it is reccomended to not store for more than one month, but actually it can be stored for several months. For longer periods (years) I would prefer to purify, suspend in TE and store at -20 or even better at -80C.
PCR product is DNA and will keep for a VERY long time... I'd simply freeze the reaction tubes. No worries!
Good Luck
G
Just make sure you store it in a good place in the freezer (not in the door where it could thaw).
One of my colleagues was hurry at weekend, forgetting he put it in his hand bag instead of putting in freezer and next week he found it OK. I put it at 4 C for more than a month and found OK. -20 C is nice for several months but put it in deeper in the freezer if you want to forget it for several years.
Like you can store purified DNA at 4degress or -20degrees for months, the same way you can store PCR prodcut also. Sequencing won't be affected by that as DNA is very stable.
PCR products are incredibly stable - even more so than genomic DNA. I've had products that were stored at room temp in a bench drawer for several years which I was able to both direct sequence and TA-clone with no problems. The only thing to maybe avoid is multiple freeze-thaw cycles (which can cause shearing), so if you're going to be using it a lot in a relatively short time period it's probably better to go with 4oC refrigeration instead of freezing. Although even there it's more a problem with genomic DNA than PCR products.
Accidentaly I leave pcr products during three days at room temperature (19°C) and it was sequenced after without any problem, PCR products are very stable
Yes you can store for long time as much as you can @ -20 to -50 degree, during this also avoid freezing and thawing cycles, this might influence your product.
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