Megha Sah

3 Questions 8 Answers 0 Followers

Questions related from Megha Sah

How can I effectively release activated small G-Protein from heterologous cell pellet to carry out an activated protein profiling?

I am trying to study the activation profile of a small G-Protein upon treatment with various kinase inhibitors. To assess the activation profile of this G-protein we employ a protein pull down...

07 July 2015 9,455 2 View

What is the best reagent to use for increasing intracellular calcium levels in heterologous HEK cells?

This is the protocol I have been using (and struggling with) -prior to treatment with 2.5 uM Thapsigargin (A SERCA blocker) or 1uM Ionomycin (a calcium ionophore), the cells are starved for 24...

02 February 2014 4,185 4 View

Why is my protein of interest getting stuck at the stacking and separating gel interface?

180 kDa protein is getting stuck at the stacking gel and separating gel interface. Stacking gel-3.5% Separating gel-12.5% (this percentage is chosen because we run a 18kDa protein along with the...

02 February 2014 1,938 6 View