What should I do for my controls not to stain my secondary antibody ( IHC for mice muscle) ?

I am staining mice muscle for macrophages (CD68) and lymphocytes (CD3). The challenge that am facing is that my control is also staining with the secondary antibody even though I did not add the...

15 October 2020 8,139 3 View

Triangulation of data sources in qualitative research: Do you consider it mixed methods research and why, whether its a yes or no?

It is common in qualitative research to interview for example patients and their partners or their health professionals to talk about the same topic and explore the perspectives of all the people...

30 July 2020 538 21 View

How do i determine the sample size for a study looking at the treatment outcomes of mental health patients in a community house ?

How do i determine the sample size for a study looking at the treatment outcomes of mental health patients in a community house that is a step down from inpatient unit. The study will look at...

12 May 2020 7,072 3 View

Who can link me to Cost / Benefit Analysis of Treatment Options for Produced Water of varying quality and quantity?

I work in the oil industry and I wish to focus on reducing reinjection of "free" (non-water right controlled) produced water for beneficial use where economics dictate feasibility. Who can direct...

24 February 2020 3,059 2 View

How is confirmatory factor analysis not just a tautology?

Hello, very new to confirmatory factor analysis (CFA) and structural equation modeling, and I'm trying to understand how to think about confirmatory factor analysis. Suppose I have a construct,...

11 November 2019 3,330 4 View

What can I use to enrich my media if am screening for urease producing microorganisms?

I got a soil sample and I want to screen for urease producing bacteria, what can I add to the medium in other to enhance the urease producing bacteria?

03 July 2019 9,846 4 View

How to deal with member check conflicts in qualitative research?

Member checking or participant checking is done to validate and improve the credibility of quallitative research results. I am planning to ask my participants to help with that (will ask them to...

27 May 2019 7,799 13 View

How do you create a calibration curve for H2O2 ?

Specifically I'll be looking at H2O2-titanium complexes (in plant tissue) and will be measured @ 415nm. I'm wondering how I can create a calibration curve for the complex... I have 30% H2O2 and...

13 December 2018 4,432 3 View

Improvised abacus? Are there Latin references to betting at Roman amphitheatres, or modern references to Roman use of abaci? Or was it a gaming board?

11 June 2015 1,010 68 View

Do we have instrument development experts among rehabilitation professionals in Sub-Saharan Africa?

It has been noted that clinicians in Africa are forced to use instrument measures that were developed and validated either for American and European children which are quite limited. It is...

01 January 1970 528 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View