Abnormal notched curve in qPCR ?

Dear all, Any comment on the reasons of appearance of notched qPCR curves ? See attached. The machine in Roch LC480

09 October 2018 1,922 0 View

Abnormal notched curve in qPCR ?

Could You help explaning the reasons why a notched curve after qPCR appears ? The machine is Roch

09 October 2018 5,951 0 View

Which tool to be used for building miRNA-mRNA interaction ?

I have a list of miRNAs and a list of predicted target mRNAs. The mRNA targets were created by other ways. I am searching for a tool to test whether an interaction (binding) could exist between...

08 September 2018 6,148 4 View

Speculation about virus titers in a disinfection study for un-measured contact time ?

Dear All, Could any body help me in finding the best statistical/model to see the un-measured titres of virus in a time points that extend beyond the ones I measured. For instance I am testing...

07 August 2018 3,735 2 View

In gene bank submission, what is the reason of "your seq. contains stop codons" ?

I am trying to submit sequences of COX-1 gene(mitochondrial gene) to gene bank. My sequences is almost 100% identical to a published one. It return a message saying that they contain stop codons...

04 May 2018 984 6 View

Which type of regression to be used ?

What is the suitable regression to be used if I have binary independent variables and also the dependent variables ar binary ? i.e. yes/no ? Details: I am checking whether the presence/absence of...

09 October 2017 634 6 View

Any help regarding small nucleolar RNAs (SnoRNAs) ?

08 September 2015 9,087 1 View

Why do I loos some DE miRNAs upon combining some time points together during the DE analysis?

I have a time series experiment and want to compare DE miRNAs between these time points and the Control. I realized that I loos some DE miRNAs upon combining some time points together and conduct...

08 September 2015 1,466 1 View

How can one determine miRNAs in ducks and Turkey ?

04 May 2015 946 2 View

Can I use U6 primer assay from Qiagen as standard gene in miRNA detection in chicken samples ? Both are 100 % conserved ?

04 May 2015 3,231 1 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View