Manan Mitra

3 Questions 5 Answers 0 Followers

Questions related from Manan Mitra

Could anyone recommend a protocol to estimate the concentration of residual antibiotics in a tissue allograft?

I have been trying to detect and quantify residual antibiotics left over from processing of my tissue allografts. Could anyone help me with the same?

02 August 2019 8,598 4 View

How do I remove PCR inhibitors from a DNA elute?

I am currently extracting DNA from dried blood spots using the QIAamp DNA blood mini Kit. So far, the DNA concentration of my elute has been ~2-5 ng/uL with a 260/280 ratio of 1.8-2.5. Upon...

25 April 2018 9,189 9 View

Why is there no PCR gene amplification on the DNA isolated from dried blood spots?

I have isolated gDNA from human dried spots with a yield of 13.8ng/uL and a purity (260/280) of 1.82. However I observe that these isolated DNA is not amplifying in PCR. Can anyone tell me why...

16 April 2018 1,114 8 View