Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

How Healthcare Personnel help frontline workers cope with stress and mental burden during COVID-19?

Which methods does they suggest to ease the burden and stress as a teamwork?

09 December 2020 9,605 7 View

Which are the drawbacks of using a medical CT for scanning fossils?

I am in the need of scanning some fossils for analyzing their inner structure. Unfortunately I don't have access to a proper CT or micro-CT of industrial use that suits nicely fossils and neither...

16 November 2020 1,748 6 View

Why appear two bands one of my expected protein and another around 17kDa?

i have two bands in my western blot, i used histag antibody and HRP as secondary antibodie, the samples are recombinant proteins in ecoli, someone toldme that extra band is an heterogeneus...

10 June 2019 5,591 3 View

Does Promega's Passive Lysis 5X Buffer contains anti-protease and anti-phosphatase components?

Hi everyone, Do you know if the Passive Lysis 5X Buffer that Promega produce has these components? I always use it to lysate transfected cells and measure lucierase activity and now I'm wondering...

03 September 2018 1,322 1 View

How to set numerical data in quartiles and analyze differences of other variables between them on SPSS?

27 June 2017 6,177 2 View

Can Inferential Statistics be carried out in a Retrospective Study?

In a retrospective study based on reviewing clinical files, should I run inferential statistical tests even if no power calculation could be made to determine the required sample size before the...

01 June 2017 8,880 3 View

QRTPCR amplification of target with low GC content?

Hey everyone. I have a question. I'm doing the in vitro silencing of a small RNA of 79 bp (a snoRNA specifically), and I need to design a protocol for the detection of the known-down of this RNA....

26 December 2016 6,061 2 View

Can anybody familiar with "distance-based Moran's eigenvector map analysis" provide advice on how to do it? Also any suggestion for software on this?

Recently, studies have demonstrated that the use of Mantel test is not corrent when detecting spatial structure or spatial correlation between two multivariable data sets (community structure and...

30 May 2016 9,790 7 View

I have the next code in MATLAB and I need to parallelize it, is a Turing criteria about the Schnakenberg model?

I have the next code in MATLAB and I have a GPU and I would love to parallelize it in order to get faster results and I justo don't know how to visualize it in real time like a movie. Is there...

27 December 2015 1,126 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

Which Journals would you advise to submit a Letter which briefly talks about neurosurgical treatment of neuro-oncological patient during Sars-COV-2?

Possibily not an open access journal which does not add any publishing costs once being accepted. Thank you in advance

09 February 2021 717 6 View

How much should I centrifuge cells in agarose-coated plates to get spheroids?

Right now I am using HT29 cells, but I will also use HCT116 and probably breast cancer cell lines.

09 February 2021 2,063 6 View