Correlation coefficient between gene and protein expression??

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What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

What are the harmful effects of these extreme rainfall on the health of newborns? What are the reasons for that?

Extreme rainfall associated with climate change is causing harm to babies in some of the most forgotten places.

01 March 2021 5,647 4 View

How to predict the reaction pathway for electro organic synthesis?

Can anyone recommend some any software/models available that can predict substance reactivities and the reaction yield under a specific set of reaction conditions process and proposing pathways to...

17 February 2021 2,383 3 View

How compare between threshold value and mean gray grayscale image in python ?

Iam working on signature image and I need to find geometric features like(slant,pressure,size,baseline)

15 February 2021 7,565 2 View

What is the best spectrophotometric method to determine sulfa drugs?

There are many reagent used to determine sulfa drugs, which One give the best test

04 January 2021 3,895 5 View

What are the advantage and disadvantage of using (demographic, ECGs, CT, and MRI) datasets in cardiovascular diseases and which one is better?

there are many types of datasets that are used to predict cardiovascular diseases (CVDs) which confuse any beginner in the field. the question is which one is better in predicting CVDs and...

04 November 2020 6,282 3 View

What are some of your favorite mixed-methods studies related to environmental management and citizen science/stewardship?

I'd like to learn more about interesting environmental management and citizen science/stewardship studies that combine both quantitative and qualitative methods.

25 May 2020 4,994 1 View

What are the best ways to visualize codes with demographics information using NVIVO 12 Software?

The data coding process using NVIVO 12 software is relatively straightforward, but I'm having difficulty using the hierarchy charts to visualize the information in terms of demographics (e.g.,...

08 May 2020 4,155 3 View

How to select particules by size prior colocalization in ImageJ?

Dear all, we need to select particles (protein inclusions) by size before running a colocalization plugin (Coloc2 and/or Jacop) in ImageJ. We thought about using ComDet, create a mask from...

31 March 2020 2,674 3 View

Which better to use for conducting qualitative research; a structured interview or semi-structured interview?

structured interview vs semi-structured interview

31 March 2019 8,734 31 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View