I am just a beginner in cell culture. I opt HeLa cells as the first.
As of now, I know, to culture any cells, we need media, antibiotics and many other reagents. However, depending on each cell type above mentioned reagent condition changes. For HeLa cells, what is the best reagent conditions is acceptable? I have found tons of document to learn from. Eventually, some are conflicting with each other. For example, some says DMEM (High Glucose, L-Glutamine)+10%FBS is suitable, Some says DMEM (High Glucose+Pyruvate) is suitable, and some says EMEM is suitable.
I would highly highly appreciate all your inputs, comes from your research experience and expertise.
N.B.: I will need HeLa cells for not to perform any core biological study but for Biomedical Imaging purpose.
Hi Sandeep
Hela Cells can be grown easily in EMEM as well as in DMEM. I have worked with DMEM (+glucose + L-Glutamine) and of course with 10% FBS) and 0.5% Antibiotic antimycotic. Additionally u can supplement with Sod Pyruvate though glucose is enough. And since u r new to cell culture so just a friendly advice to keep everything aseptic and watch your handling.Here are some useful links. Hope it helps!!
https://www.cellbiolabs.com/sites/default/files/AKR-213-gfp-hela-cell-line.pdf
https://darchive.mblwhoilibrary.org/bitstream/handle/1912/8720/Cell%20Culture%20Protocols%2C%20HeLa%20and%20CHO%20cells.pdf?sequence=1
https://www.hela-transfection.com/cell-culture-information/
Thanks, Olivier Dubey , and Vikrant Mehta for making things clear and easy to deal with. I appreciate your help.
- Badges
- Science method