Correct HPLC column?

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Black dots "bubbling" at cell surface?

Hi, I observed a strange behaviour in my cell lines I had never seen before. Those are stable lines from Ovarian cancer (line's name: HEY) and from Prostate cancer (line's name: PC-3). Both lines...

08 April 2024 1,418 0 View

LCA calculation on raw materials textile use ?

Dear friends, I am Stefano Cipriani and dong an ecolabel that will be useful for the market, especially to increase use of recycled materials. I have a question that would love to share here in...

27 March 2024 5,298 3 View

The twin-effect in water cannot be different than the one in vacuum, what are the consequences on Lorentz Transformations?

Consider two clocks set just under the surface of water, they are synchronized. One of them departs at velocity v and then rejoins at -v. The overall time dilation must be the same as the one in...

14 March 2024 2,203 86 View

Does BFA keep ROS inside of cells?

I recently discovered that BFA is used in flow cytometry, enabling the observation of intracellular cytokines that would be otherwise released. Is the same principle applicable to ROS? I would...

04 March 2024 7,094 1 View

Can ip E.coli injection cause death in 4 hours?

Does an ip E.coli injection (10^8 ) cause death in 4 hours in C57BL/6? Do I need to hydrate the mouse? Is this a good concentration to mimic a septic situation?

04 March 2024 4,552 1 View

How do the power-split and compound architectures of hybrid electric vehicles (HEVs) work?

I'm searching for a comprehensive textbook or paper on power-split and compound architectures in hybrid electric vehicles (HEVs). I've come across some very interesting papers on HEVs. However,...

11 February 2024 8,516 0 View

Micrometer calibration reference method?

Good day, may you suggest a reference method for micrometers calibration? Thanks in advance Stefano

06 February 2024 8,027 3 View

Could anyone help to identify this TC contamination?

Hi ResearchGate community, can anyone help us out with this contamination? We work with iPSC-NPC. This contamination happens ONLY when we put the neuronal progenitors in coverslips + media for...

14 December 2023 6,435 0 View

What is the subsample size for a confusion matrix?

Dear all, do you know what is the correct subsample size (number of pixel) to carry out a confusion matrix? I am digitizing the true classes on an image to check the accuracy of the classifier....

11 August 2023 6,528 2 View

Has anyone had problems with the PyroMark Q48 cartridges?

Hi everyone, a few months ago we bought the PyroMark Q48 to replace the old Q96, but we used it very little due to continuous malfunctions of the cartridge with enzyme and substrate. We've already...

23 July 2023 8,854 0 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Why is electronic energy 0.000 for liquid crystal compounds and is invariable with temperature in Guassian 09 software?

Are there any suggestions or insights you can provide to help address this problem

06 August 2024 1,443 2 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

How can we use mobile apps for improving students' academic performance?

Mobile apps can be a powerful tool for enhancing academic performance, how can we use mobile apps for improving academic performance

04 August 2024 9,492 0 View

Why is TLC not a form of partition chromatography?

Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?

03 August 2024 7,035 3 View

Is there any Indian Company or Startups, from any universiy or IITS, which manufacture Gas Chromatography instrument , i want to make a custOmized G?

or we can collaborate

01 August 2024 6,705 2 View