i believe its 7018.2 g/mole
( 5'- rUrCrA rCrCrG rGrGrU rGrUrA rArArU rCrArG rCrUrU rG -3' )
Please can someone explain clearly how i can convert this?
Hi Danielle
you'll need further information to convert that. you'll need the molecular weight of the cel-mir-39.
fred
ok
then, your stock is:
1600 fMol/ul = 1600x10-15 Mol/ul = 1600x10-15 * 7018.2 g/ul = 11229120x10-15 g/ul = 11.23 ng/ul
you then need to go from 11.23 to 2 ng/ul:
for instance, add 1ul of your stock to 4.615ul of pure water to get a 5.615 ul solution at 2ng/ul...
on higher volumes of course the results will be better, to address the pipetting issues.
all the best
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