Danielle Lewis

7 Questions 8 Answers 0 Followers

Questions related from Danielle Lewis

I have a problem with my miRNA rt qPCR kits! i dont believe its contamination at this stage, is there something else going on?

Using a standard rt qPCR kit. When looking at non human miRNA species there is a sort of 'background level' of expression in my results when there shouldnt be - i have tried running samples of...

11 February 2024 3,804 4 View

Does anyone here work with designing primers for sncRNA?

I would like to have some more information for primer design for tRNA's, miRNAs and other sncRNAs. I have used sRNAPrimerDB and mirPrimer ( for miRNA) before - are these reliable sources? Any...

21 July 2023 8,002 1 View

Converting ng/ul of RNA to fmol/ul - help please :) ?

Need 2ng/ul RNA ( from cel-mir-39) Our stock is at 1600 fMol/ul i believe its 7018.2 g/mole ( 5'- rUrCrA rCrCrG rGrGrU rGrUrA rArArU rCrArG rCrUrU rG -3' ) Please can someone explain clearly...

24 May 2023 325 3 View

Does anyone have experience working with Total RNA spike - ins for total RNA extraction & miRNA quantification with rt - qPCR?

Does anyone have experience working with Total RNA spike - ins for total RNA extraction & miRNA quantification with rt - qPCR? I have been made aware of the c.elegans spike in however i would...

22 November 2022 9,182 0 View

Do you use qpcr to confirm biomarkers in ngs or do you use ngs to confirm qpcr results ?

Which way round for biomarker discovery?

11 November 2022 6,193 2 View

Opinions please- best storage of plasma for total RNA extraction?

Freezing with RNA/DNA Sheild?

11 October 2022 9,628 2 View

Is it possible to extract RNA from blood using trizol extraction method?

I am aware that whole blood is not great for extracting RNA. But basically i need dto do it. I have found a basic protocol for the trizol method with whole blood however im not convinced my...

11 November 2019 7,080 4 View