Are my cells contaminated with mycoplasma?

I suspect contamination with mycoplasma and fixed the cells with 4%PFA and stained them with Dapi. This is the image I got, I usually don't get any of those small rounded dapi foci outside the...

06 August 2020 6,412 1 View

Do you think improved varieties and better management practices will be the key to future increases in food production?

Agricultural experts generally agree that future increases in food production will come from three options:1) expanding the land area, 2) increasing the frequency in which the land is cropped, and...

03 July 2020 8,215 4 View

Do you think Golden Rice will alleviate hunger and malnutrition, especially in developing countries?

Golden Rice is a GMO rice plant that was modified to produce beta-carotene (provitamin A) in its grain. It was invented by the German scientists Prof. I. Potrykus (ETH) and Prof. P. Beyer (Univ...

20 June 2020 8,154 54 View

Is sustainable agriculture the same as ecological agriculture?

"Sustainable agriculture" and ecological agriculture" are two very popular terms for both government and non-government workers. Many people use them interchangeably thereby causing a lot of...

01 June 2020 1,624 100 View

What is the explanation for the "no correlation" between soil test and plant tissue test values?

In our recent research on sweetpotato here in Leyte, Philippines, we have observed a no correlation between soil test and plant tissue test values for N, P and K. Marschner (1995) in his classic...

27 April 2020 5,357 18 View

Purification of histag protein using HISTRAP HP (GE). What is the best lysis buffer and which protocol may be most suitable?

Purification of histag protein using HISTRAP HP (GE). I am working with insect cells (Tn5B) and my antigens are expressed in the nucleus. I am having difficulty lysing the cells because we have no...

10 March 2020 8,609 2 View

How to estimate the penetration thickness of light in a solid sample?

I have a solid sample and I need to determine the depth of light penetration to produce a pellet and elucidate some optical phenomenons.

27 May 2019 3,067 1 View

Where can I find information about contractual practices that stimulate performance in urban transport by bus?

I would like to learn more about the contract design of urban transport by bus that stimulate competition and better performance. Among the elements that I would like to learn more about...

02 April 2019 7,562 1 View

How can I determine the method from a .raw file?

I have raw files from a ltq-velos orbitrap but I would like to know the running method used, for instance the resolution at ms1 and ms2. (Proteomics/mass-spec)

22 August 2018 6,887 4 View

No title

15 March 2018 4,243 7 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Is there an open source free software for text mining and sentiment analysis?

I am looking for a free software for text mining and sentiment analysis for my research on customer review mining (it involves calculating polarity of attributes,opinion oriented information...

25 February 2021 1,920 6 View

Suggest me some trending topics for phd research in text mining/AI/machine learning?

My master's research is in info retrieval and text mining. I would be grateful if you could help me to select a good topic for my phd research proposal.

24 February 2021 393 14 View

How can I simulate the optical absorption spectrum of a biomaterial (like DNA or protein) in COMSOL?

I need to simulate the optical properties of a biomaterial, namely DNA, in COMSOL. Basically I need to simulate the IR absorption spectrum of a cylinder filled with a 'concentration' of DNA. I am...

21 February 2021 3,043 3 View

What is/are the best ML classifier(s) for Sentinel2 images?

Hi I am trying to segment a sentinel2 image. At this stage, I want to run a binary classifier that assigns each pixel to either farm or non-farm pixel. For this purpose, I have 4 10m bands...

15 February 2021 3,596 9 View

Can you remove a publication from me?

I thought it might be mien from decades ago when I did research in math, but it is not mine.

03 February 2021 7,712 4 View

No title

Connecting With the New Digital Customer Immersive technologies

02 February 2021 614 9 View

Is Handheld XRF analyzer useable? and accurate in geochemical test?

What is the different between Lab XRF and Hanheld XRF, is it accurate and useable to analyze sedimentary rocks?

23 January 2021 9,941 6 View