Conditions:
1. Cultures should be healthy with a viability of >90% and no signs of microbial contamination.
2. Cultures should be in the log phase of growth.
3. For a high concentration of serum/protein: 20-90%, what is the appropriate concentration?
4. Use dimethyl sulphoxide (DMSO) or glycerol as cryoprotectant to help protect the cells from rupture by the formation of ice crystals. The most commonly used cryoprotectant is DMSO at a final concentration of 10%, but is it appropriate for all cell lines?
5. Slow freeze and quick thaw. Storage: The vials were held overnight at −80°C, then transferred to liquid nitrogen for long-time storage; thaw quickly by incubation in a 37°C water bath for 3-5 minutes.
Are these conditions appropriate for all cell lines? Are there any additional points?
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