How to find data for Depreciation Rate or Net Domestic Product?

I'm currently working on the paper The Green Solow model of William A. Brock and M. Scott Taylor. In order to create my models, I'm currently seeking for a database where one can find the...

24 January 2021 5,342 4 View

How do we use polarization in Gromacs 2019?

It seems that polarization has been implemented in the Gromacs version of 2019 and further. However, I can't seem to find relevant info in the documentation except for the edits that have to be...

30 June 2020 1,835 3 View

How to calculate average with only valid variables in Stata (version 14)?

I'm trying to calculate an average of nine different numerical variables as a new variable in Stata (version 14). Some of these nine variables have missing values. I would like Stata to divide the...

25 March 2020 6,583 2 View

Any software piloting MWG AG Biotech Primus HT Multi Block PCR-System ?

Hi, Could someone share the software piloting the MWG AG Biotech Primus HT Multi Block PCR-System ? it is an old PCR system and our computer crashed and with it, the software. thanks a lot

25 September 2019 7,025 1 View

I need any solution for PVT datas fiting of molten polymers to several equation of state therories? FOV Theory or other ?

For a project on interfacial/surface tension, we performed PVT experiments on several polymer melts. To adjust PVT data to surface tension, i first need to fit my PVT data to the FOV equation of...

19 June 2018 4,599 1 View

DNA samples mixed up, wrong sequencing identification, how to solve it?

We sent for sequencing several bacterial strains in batch to an external provider. When we got our results back and started to analyze them, we found out that several of our samples were not what...

22 May 2018 5,533 5 View

How do you properly critique a research article whose major results are significantly dependent on a software (where you just input data then run) ?

Many research outputs have their major results run by a software. Relying on the results of these software applications just because they are commonly used in that certain topic of research, seems...

03 May 2018 4,336 7 View

Is it alright to generalize optimum nutrient levels/ratios from researches tackling the same dinoflagellate species but different morphotypes?

19 April 2017 9,911 1 View

Technique for DNA extraction from cold water corals polyps (Lophelia pertusa)?

Does anyone have experience with DNA extraction from polyps? I would like to look at the microbial community and I am unsure how to handle the mixture hard coral and soft tissue. Thanks for any...

26 January 2016 3,873 2 View

What are the precise sequences for Age1 site to add to oligos for shRNA cloning in pLKO.1?

29 March 2015 9,285 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View